Transgenic Fish. • Generally genetic modification entails manipulation of DNA . GMOs also are involved in controversies over GM food with respect to whether using GM fish as safe is safe, whether it would exacerbate or cause fish allergies, whether it should be labeled, and whether GM fish and crops are nee… Transgenic fish (GM fish) are considered in terms of the history of their production, with molecular details of the genes used and the modifications obtained, and the present and future contributions of transgenic fish to scientific research, medicine, and aquaculture. Scientist in India has developed experimental transgenic of rohu fish, zebra fish, catfish and singhi fish. 3. • Aim is to introduce a new trait to fish • GM fish has been approved by FDA. The most established method for gene transfer in fish is microinjection. Transgenic zebrafish are powerful tools to analyze photoreceptor morphology, cell biology, and regeneration. Critics have objected to use of genetic engineering per se on several grounds, including ethical concerns, ecological concerns (especially about gene flow), and economic concerns raised by the fact GM techniques and GM organisms are subject to intellectual property law. The use of the microinjection method results in higher survival rates for manipulated fish embryos than the electroporation method. TRANSGENIC FISH • Transgenic fish or genetically modified fish (GM fish) are genetically modified organism. To date, studies have utilized transgenic technology to express intrinsic proteins behind very strong promoters (e.g., Xenopus opsin or cone transducin promoters). From: Encyclopedia of Fish Physiology, 2011. Microinjection method:Microinjection method has been used successfully in the production of transgenic fish and is a commonly used technique due to its simplicity and reliability. The DNA of the fish is modified using genetic engineering techniques. Microinjection that allows delivery of the transgene directly into the nucleus, Transgene is directly microinjected into the male pron… Genes, promoters and vectors of indigenous origin are now available for only two species, namely rohu and singhi for engineering growth. Related terms: Wild Type

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